Electrochemical definitions of O2 sensitivity and oxidative inactivation in hydrogenases.

Journal article

A new strategy is described for comparing, quantitatively, the ability of hydrogenases to tolerate exposure to O2 and anoxic oxidizing conditions. Using protein film voltammetry, the inherent sensitivities to these challenges (thermodynamic potentials and rates of reactions) have been measured for enzymes from a range of mesophilic microorganisms. In the absence of O2, all the hydrogenases undergo reversible inactivation at various potentials above that of the H+/H2 redox couple, and H2 oxida...

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Authors: Vincent, K; Parkin, A; Lenz, O; Albracht, S; Fontecilla-Camps, J

• Publication status: Published
• Journal: Journal of the American Chemical Society
• Volume: 127
• Issue: 51
• Pages: 18179-18189
• Publication date: 2005-12-01
• DOI: 10.1021/ja055160v
• EISSN: 1520-5126
• ISSN: 0002-7863
• Language: English
• Keywords: Hydrogen; Thermodynamics; Oxygen; Enzyme Activation; Electrochemistry; Hydrogenase; Anaerobiosis; Oxidation-Reduction