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Expression, purification and crystallization of Aspergillus nidulans NmrA, a negative regulatory protein involved in nitrogen-metabolite repression.

Abstract:

The NmrA repressor protein of Aspergillus nidulans was overproduced in Escherichia coli and purified to homogeneity. Gel-exclusion chromatography showed that NmrA was monomeric in solution under the buffer conditions used. The protein was crystallized in three forms, belonging to trigonal, monoclinic and hexagonal space groups. Two of these crystal forms (A and B) diffract to high resolution and thus appear suitable for structure determination. Crystal form A belongs to space group P3((1))21,...

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Publication status:
Published

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Publisher copy:
10.1107/s090744490101410x

Authors


Nichols, CE More by this author
Cocklin, S More by this author
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Journal:
Acta crystallographica. Section D, Biological crystallography
Volume:
57
Issue:
Pt 11
Pages:
1722-1725
Publication date:
2001-11-05
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
URN:
uuid:21ea262d-d203-4f1b-bd3c-b1dd0c4c28b6
Source identifiers:
72606
Local pid:
pubs:72606

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