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Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase.

Abstract:

delta-(L-alpha-Aminoadipoyl)-L-cysteinyl-D-valine (ACV) synthetase was isolated and partially characterised from Cephalosporium acremonium CO728 and Streptomyces clavuligerus. The purification procedure resulted in a 745- and 277-fold increase in specific enzyme activity, respectively. Both enzymes had similar apparent molecular masses of ca. 300 kdaltons by SDS-polyacrylamide electrophoresis, under reducing and denaturing conditions, and in excess of 600 kdaltons in the native state by gel f...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Journal of antibiotics More from this journal
Volume:
44
Issue:
2
Pages:
241-248
Publication date:
1991-02-01
ISSN:
0021-8820
Language:
English
Keywords:
Pubs id:
pubs:35058
UUID:
uuid:21cc7b0c-97f7-490f-9506-08eb493d5189
Local pid:
pubs:35058
Source identifiers:
35058
Deposit date:
2012-12-19

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