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Determining the stoichiometry and interactions of macromolecular assemblies from mass spectrometry.

Abstract:

The growing number of applications to determine the stoichiometry, interactions and even subunit architecture of protein complexes from mass spectra suggests that some general guidelines can now be proposed. In this protocol, we describe the necessary steps required to maintain interactions between subunits in the gas phase. We begin with the preparation of suitable solutions for electrospray (ES) and then consider the transmission of complexes through the various stages of the mass spectrome...

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Publication status:
Published

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Publisher copy:
10.1038/nprot.2007.73

Authors


Hernández, H More by this author
Robinson, CV More by this author
Journal:
Nature protocols
Volume:
2
Issue:
3
Pages:
715-726
Publication date:
2007
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
URN:
uuid:2158c8d2-2145-4f02-85b4-70bcc29f6b9f
Source identifiers:
59299
Local pid:
pubs:59299

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