- Abstract:
-
The growing number of applications to determine the stoichiometry, interactions and even subunit architecture of protein complexes from mass spectra suggests that some general guidelines can now be proposed. In this protocol, we describe the necessary steps required to maintain interactions between subunits in the gas phase. We begin with the preparation of suitable solutions for electrospray (ES) and then consider the transmission of complexes through the various stages of the mass spectrome...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1038/nprot.2007.73
-
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- Journal:
- Nature protocols
- Volume:
- 2
- Issue:
- 3
- Pages:
- 715-726
- Publication date:
- 2007
- DOI:
- EISSN:
-
1750-2799
- ISSN:
-
1754-2189
- URN:
-
uuid:2158c8d2-2145-4f02-85b4-70bcc29f6b9f
- Source identifiers:
-
59299
- Local pid:
- pubs:59299
- Language:
- English
- Keywords:
- Copyright date:
- 2007
Journal article
Determining the stoichiometry and interactions of macromolecular assemblies from mass spectrometry.
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