Researchers have a growing need to push optical microscopy beyond the diffraction limit to answer key questions in biology, and stimulated emission depletion (STED) has proven to be a fluorescence imaging technique that can accomplish this goal. Biologists are currently seeking to connect molecular behavior to macroscopic behavior, determining how cells signal with each other, and how signaling at the cellular/organism level is then relayed back to DNA/RNA level control to regulate single gen...