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Dataset

Serial histology and blockface images, mouse heart, 300 sections

Subtitle:
Picro Sirius Red histology data with corresponding 0º (regular) and 55º (Brewster angle) blockface images
Version:
v1.0

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Department:
Heart Science Centre, National Lung and Heart Institute, Imperial College London, Harefield UB9 6JH, UK.
Role:
Creator
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Department:
Department of Physiology and Biophysics, Dalhousie University, Halifax, NS B3H 4R2, Canada
Role:
Creator
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Department:
Institute for Experimental Cardiovascular Medicine, University Heart Centre Freiburg – Bad Krozingen, School of Medicine, University of Freiburg, Elsässer Str 2Q, 79110 Freiburg, Germany.
Role:
Principal Investigator, Creator
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Department:
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK.
Role:
Depositor, Creator, Researcher

Contributors

Department:
BHF Experimental MR Unit, Division of Cardiovascular Medicine, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.
Role:
Principal Investigator
Department:
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK.
Role:
Principal Investigator
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Grant:
NH/13/30238
Funding agency for:
Project
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Grant:
FS/11/50/29038
Funding agency for:
Jürgen E. Schneider
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Grant:
FS/12/17/29532
Funding agency for:
Peter Kohl
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Publisher:
Institute for Experimental Cardiovascular Medicine, University of Freiburg; Institute of Biomedical Engineering, Department of Engineering Science and Wellcome Trust Centre for Human Genetics, University of Oxford; Heart Science Centre, National Lung and Heart Institute, Imperial College London
Publication date:
2016
DOI:
Data collected:
2014-10-04 - 2015-03-07
Keywords:
Subjects:
Format:
Digital
Documentation:
<p>Mouse hearts were excised after Schedule 1 culling according to the UK Home Office guidance on the Operation of Animals (Scientific Procedures) Act of 1986, washed swiftly in 37°C normal Tyrode solution (NaCl 140 mM; KCl 5.4 mM; MgCl2 1 mM; HEPES 5 mM; Glucose 10 mM; CaCl2 1.8 mM; pH 7.4, 30010 mOsm) containing heparin (10u/ml) and then cannulated in cold Tyrode solution with heparin in order to perfuse the coronary vasculature from the aorta. Afterwards, hearts were arrested using modifi... Expand documentation

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