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Sugar-coated sensor chip and nanoparticle surfaces for the in vitro enzymatic synthesis of starch-like materials

Abstract:

The insoluble glucan polymer starch is a major player in the human diet; it is also an important bulk commodity. Nonetheless, our understanding of its biochemistry remains poor, not least because of the challenge of analysing enzymes that operate across the solid–liquid interface. In the present study, the enzymatic polymerisation of glucans immobilised on gold sensor chip and nanoparticle surfaces was achieved with Arabidopsis phosphorylase AtPHS2. The basis of the action of AtPHS2 on surfac...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1039/C3SC51829A

Authors


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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
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Grant:
Institute Strategic Programme Grant on Understanding and Exploiting Metabolism (MET) [BB/J004561/1]
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Grant:
Marie Curie Early Stage Training grant [MEST-CT-2005-019727]
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Science Journal website
Volume:
5
Issue:
1
Pages:
341-350
Publication date:
2014-01-01
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
URN:
uuid:1fcd3e5a-09fc-47dc-9232-a2c54c006af4
Source identifiers:
612897
Local pid:
pubs:612897

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