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Journal article

Single-molecule FRET analysis of protein-DNA complexes.

Abstract:
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.

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Authors


Heilemann, M More by this author
Lymperopoulos, K More by this author
Kapanidis, AN More by this author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
543
Pages:
503-521
Publication date:
2009
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:1f17580e-571c-4669-9427-3ee4b8d659c7
Source identifiers:
2550
Local pid:
pubs:2550

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