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Journal article

Single-molecule FRET analysis of protein-DNA complexes.

Abstract:
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.

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Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
543
Pages:
503-521
Publication date:
2009-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Source identifiers:
2550

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