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Deep sequencing of hepatitis B virus (HBV) genomes using rolling circle amplification and Nanopore

Abstract:

The optimisation of unbiased deep-sequencing methods for full-length HBV genomes will inform detailed insights into the evolution and diversity of this pathogen. Nanopore-based sequencing technologies offer the potential for real-time detection and sequencing of blood-borne viruses, useful for clinical diagnosis, drug-resistance calling and high-resolution molecular epidemiology. To date, sequencing accuracy has limited the full potential of Oxford Nanopore’s long-read capabilities. We here d...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1016/S0168-8278(18)31789-6

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; NDM Experimental Medicine
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; BDI-NDM
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Human Genetics Wt Centre
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Human Genetics Wt Centre
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Publisher:
Elsevier Publisher's website
Volume:
68
Issue:
S1
Pages:
Article: S762
Publication date:
2018-04-23
Acceptance date:
2018-01-25
DOI:
Pubs id:
pubs:859546
URN:
uri:1f0a2361-4928-4a6e-b0c1-1a3c7dd4d216
UUID:
uuid:1f0a2361-4928-4a6e-b0c1-1a3c7dd4d216
Local pid:
pubs:859546

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