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Specifically horizontally tethered DNA probes on Au surfaces allow labelled and label-free DNA detection using SERS and electrochemically driven melting

Abstract:

Controlled covalent attachment of dsDNA horizontally orientated on a gold surface is achieved through the use of a single surface-linker located approximately half way along the attached DNA probe strand. We show that horizontally oriented dsDNA on a gold surface can undergo melting and re-hybridization to target strand in solution and thus can be used for the detection of specific target DNA sequences using surface-enhanced Raman spectroscopy (SERS). We show that a range of lengths of target...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/c5sc03185k

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More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Science Journal website
Volume:
7
Issue:
1
Pages:
386-393
Publication date:
2015-10-08
Acceptance date:
2015-10-08
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Pubs id:
pubs:590222
UUID:
uuid:1ef9778b-6055-4c45-b8fd-572c34afd31d
Local pid:
pubs:590222
Source identifiers:
590222
Deposit date:
2016-03-01

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