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A real-time RT-PCR for detection of clade 1 and 2 H5N1 influenza A virus using locked nucleic acid (LNA) TaqMan probes.

Abstract:

BACKGROUND: The emergence and co-circulation of two different clades (clade 1 and 2) of H5N1 influenza viruses in Vietnam necessitates the availability of a diagnostic assay that can detect both variants. RESULTS: We developed a single real-time RT-PCR assay for detection of both clades of H5N1 viruses, directly from clinical specimens, using locked nucleic acid TaqMan probes. Primers and probe used in this assay were designed based on a highly conserved region in the HA gene of H5N1 viruses...

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Publication status:
Published

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Publisher copy:
10.1186/1743-422x-7-46

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Journal:
Virology journal More from this journal
Volume:
7
Issue:
1
Pages:
46
Publication date:
2010-01-01
DOI:
EISSN:
1743-422X
ISSN:
1743-422X
Language:
English
Keywords:
Pubs id:
pubs:92784
UUID:
uuid:1ef26525-b208-4ad5-bbb1-7a32e940274d
Local pid:
pubs:92784
Source identifiers:
92784
Deposit date:
2012-12-19

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