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Synthesis of fluorescence-labelled disaccharide substrates of glucosidase II.

Abstract:

The fluorescence-labelled disaccharides Glcalpha(1-->3)GlcalphaOR and Glcalpha(1-->3)ManalphaOR, both substrates for the glycoprotein-processing enzyme glucosidase II, were synthesised via the use of a n-pentenyl-derived linker at the anomeric position. This allowed incorporation of a pyrenebutyric acid label, via a sequence of oxidative hydroboration, mesylation, azide displacement, reduction with concomitant global deprotection, and peptide coupling. Selective activation of a fully ar...

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Publication status:
Published

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Journal:
Carbohydrate research
Volume:
338
Issue:
19
Pages:
1937-1949
Publication date:
2003-09-01
DOI:
EISSN:
1873-426X
ISSN:
0008-6215
Language:
English
Keywords:
Pubs id:
pubs:100115
UUID:
uuid:1eeb33c9-536a-4cea-ad3c-75fab4e5892c
Local pid:
pubs:100115
Source identifiers:
100115
Deposit date:
2012-12-19

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