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Towards an optimized workflow for middle-down proteomics

Abstract:

Mass spectrometry (MS)-based proteomics workflows can crudely be classified into two distinct regimes, either targeting relatively small peptides (i.e. 0.7 kDa < Mw < 3.0 kDa) or small to medium sized intact proteins (i.e. 10 kDa < Mw < 30 kDa), respectively termed bottom-up and top-down proteomics. Recently, a niche has started to be explored covering the analysis of middle-range peptides (i.e. 3.0 kDa < Mw < 10 kDa), aptly termed middle-down proteomics. Although middle-dow...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1021/acs.analchem.6b03756

Authors


Cristobal, A More by this author
van den Toorn, HWP More by this author
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Department:
Oxford, MPLS, Chemistry, Physical & Theoretical Chem
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Grant:
Roadmap Initiative Proteins@Work (project number 184.032.201); VIDI grant 700.10.429 for S.M.; OP‐Punt Grant 718.015.003 for A.J.R.H.
Publisher:
American Chemical Society Publisher's website
Journal:
Analytical Chemistry Journal website
Volume:
89
Issue:
6
Pages:
3318–3325
Publication date:
2017-02-25
Acceptance date:
2017-02-24
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
Pubs id:
pubs:682003
URN:
uri:1eb889b1-831b-48cb-bba2-28cafae76d43
UUID:
uuid:1eb889b1-831b-48cb-bba2-28cafae76d43
Local pid:
pubs:682003

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