Determination of the lifetime and force dependence of interactions of single bonds between surface-attached CD2 and CD48 adhesion molecules.

Journal article

We studied single molecular interactions between surface-attached rat CD2, a T-lymphocyte adhesion receptor, and CD48, a CD2 ligand found on antigen-presenting cells. Spherical particles were coated with decreasing densities of CD48-CD4 chimeric molecules then driven along CD2-derivatized glass surfaces under a low hydrodynamic shear rate. Particles exhibited multiple arrests of varying duration. By analyzing the dependence of arrest frequency and duration on the surface density of CD48 sites...

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Authors: Pierres, A; Benoliel, A; Bongrand, P; Van Der Merwe, P

• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 93
• Issue: 26
• Pages: 15114-15118
• Publication date: 1996-12-01
• DOI: 10.1073/pnas.93.26.15114
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Animals; Antibodies, Monoclonal; Microscopy, Atomic Force; T-Lymphocytes; Models, Chemical; Rats; Antigens, CD2; Kinetics; Sensitivity and Specificity; Antigens, CD; Mice