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Three-dimensional expansion: in suspension culture of SD rat's osteoblasts in a rotating wall vessel bioreactor.

Abstract:

OBJECTIVE: To study large-scale expansion of SD (Sprague-Dawley) rat's osteoblasts in suspension culture in a rotating wall vessel bioreactor (RWVB). METHODS: The bioreactor rotation speeds were adjusted in the range of 0 to 20 rpm, which could provide low shear on the microcarriers around 1 dyn/cm2. The cells were isolated via sequential digestions of neonatal (less than 3 days old) SD rat calvaria. After the primary culture and several passages, the cells were seeded onto the microcarriers...

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Publication status:
Published

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Journal:
Biomedical and environmental sciences : BES More from this journal
Volume:
20
Issue:
2
Pages:
91-98
Publication date:
2007-04-01
ISSN:
0895-3988
Language:
English
Keywords:
Pubs id:
pubs:61998
UUID:
uuid:1e930a9e-8da2-4289-aec6-3e137754ccee
Local pid:
pubs:61998
Source identifiers:
61998
Deposit date:
2013-11-17

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