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Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy.

Abstract:

Modern fluorescence microscopy applications go along with increasing demands for the employed fluorescent dyes. In this work, we compared antifading formulae utilizing a recently developed reducing and oxidizing system (ROXS) with commercial antifading agents. To systematically test fluorophore performance in fluorescence imaging of biological samples, we carried out photobleaching experiments using fixed cells labeled with various commonly used organic dyes, such as Alexa 488, Alexa 594, Ale...

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Publication status:
Published

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Publisher copy:
10.1039/c0cp01919d
Journal:
Physical chemistry chemical physics : PCCP More from this journal
Volume:
13
Issue:
14
Pages:
6699-6709
Publication date:
2011-04-01
DOI:
EISSN:
1463-9084
ISSN:
1463-9076
Language:
English
Keywords:
Pubs id:
pubs:196002
UUID:
uuid:1e8f1a00-4066-428e-ab4e-eb0de2886e56
Local pid:
pubs:196002
Source identifiers:
196002
Deposit date:
2012-12-19

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