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Functional complementation and genetic deletion studies of KirBac channels: Activatory mutations highlight gating-sensitive domains

Abstract:

The superfamily of prokaryotic inwardly rectifying (KirBac) potassium channels is homologous to mammalian Kir channels. However, relatively little is known about their regulation or about their physiological role in vivo. In this study, we have used random mutagenesis and genetic complementation in K +-auxotrophic Escherichia coli and Saccharomyces cerevisiae to identify activatory mutations in a range of different KirBac channels. We also show that the KirBac6.1 gene (slr5078) is necessary f...

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Publisher copy:
10.1074/jbc.M110.175687

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Journal:
Journal of Biological Chemistry More from this journal
Volume:
285
Issue:
52
Pages:
40754-40761
Publication date:
2010-12-24
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
Language:
English
Pubs id:
pubs:110366
UUID:
uuid:1e837fe5-a15e-4164-be15-a75239a19d9f
Local pid:
pubs:110366
Source identifiers:
110366
Deposit date:
2012-12-20

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