Patent

Automated glycofingerprinting strategy

Documentation:: A method of determining one or more glycosylation markers of disease comprises obtaining a diseased sample and a control sample, wherein the diseased sample is a sample from a patient diagnosed with disease and the control sample is a sample from a healthy control; releasing a diseased glycan pool of total glycoproteins from the diseased sample and a control glycan pool of total glycoproteins from the control sample without purifying the glycoproteins and without exposing the diseased sample and the control sample to hydrazinolysis wherein the total glycoproteins from the disease and the total glycoproteins from the control sample are immobilized in a high throughput format; measuring a diseased glycoprofile of the diseased glycan pool and a control glycoprofile of the control glycan pool using chromatography, mass spectrometry or a combination thereof; comparing the diseased glycoprofile and the control glycoprofiles to determine said one or more glycosylation markers of disease. A method for diagnosing and monitoring disease in a subject comprising obtaining a sample of body fluid or a body tissue of the subject; releasing a glycan pool of total glycoproteins from the sample without purifying the glycoproteins and exposing the sample to hydrazinolysis; measuring a glycoprofile of the glycan pool. Also provided are methods of glycan releasing compatible with a high throughput format and for analysis of glycosylation from 2D-PAGE spots.

Publication status:: Published

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APA Style

Dwek, R., Royle, L., Zitzmann, N., Radcliffe, C., & Rudd, P. (2006). Automated glycofingerprinting strategy. In World Intellectual Property Organization. World Intellectual Property Organization.

MLA Style

Dwek, R., et al. “Automated Glycofingerprinting Strategy.” World Intellectual Property Organization, World Intellectual Property Organization, 2006.

Chicago Style

Dwek, R, L Royle, N Zitzmann, C Radcliffe, and P Rudd. 2006. “Automated Glycofingerprinting Strategy.” World Intellectual Property Organization. World Intellectual Property Organization.
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Authors

+ Dwek, R More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Biochemistry
Role:: Author

+ Royle, L More by this author

Role:: Author

+ Zitzmann, N More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Biochemistry
Role:: Author

+ Radcliffe, C More by this author

Role:: Author

+ Rudd, P More by this author

Role:: Author

Publisher:: World Intellectual Property Organization
Journal:: World Intellectual Property Organization More from this journal
Publication date:: 2006-11-02
Patent number:: WO2006114663
Application number:: PCT/IB2005/002995
Patent status:: Issued/granted

Pubs id:: pubs:383430
UUID:: uuid:1e740364-6939-4007-8e8e-caea6ef6bf7b
Local pid:: pubs:383430
Source identifiers:: 383430
Deposit date:: 2016-02-11

Terms of use

Copyright date:: 2006

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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