Experiment Date2017-06-22 Experiment ID Notebook ID Project Experimenter Protocol Experiment Date2017-06-22 Experiment ID Notebook ID Project Experimenter Protocol Learn more about interpolating from standard curves How the data are organized
The X values are micrograms of protein. The Y values are the measured optical density. The first seven rows represent standards, where you know both concentration and signal (Y). The next three rows represent unknowns.

The goal
To fit a straight line through the standards, and then interpolate concentrations for the three unknown values.

How to fit a dose response curve
1. Click Analyze, choose Linear regression from the list of XY analyses.
2. Check the option:
Interpolate unknowns from standard curve.
3. Look at the graph to make sure the line fits nicely.
4. To find the concentrations corresponding to the unknown values, go to the results subpage: Interpolated X values. Nonlinear standard curve interpolations What if the standard curve isn't linear? saline saline saline saline con saline con saline con IL-1 IL-1 IL-1 IL-1 con IL-1 con IL-1 con Saline Plasma Saline PLasma Saline PLasma IL-1 Plasma IL-1 Plasma IL-1 Plasma Saline EVs Saline EVs Saline EVs IL-1 EVs IL-1 EVs IL-1 EVs Saline Liver Saline Liver Saline Liver IL-1 Liver IL-1 Liver IL-1 Liver 250 125 62.5 31.25 15.625 7.8125 250 125 62.5 31.25 15.625 7.8125 1.338667 0.853 0.514667 0.319667 0.214 0.160333 0.2305 0.2735 0.2955 0.3965 0.308 0.305 0.705 1.1405 0.414 0.3355 0.5315 0.434 0.0845 0.066 0.069 0.07 0.073 0.0705 0.0645 0.068 0.0685 0.069 0.1005 0.074 0.3805 0.8595 0.5 0.335 0.539 0.06 Learn more about interpolating from standard curves How the data are organized
The X values are micrograms of protein. The Y values are the measured optical density. The first seven rows represent standards, where you know both concentration and signal (Y). The next three rows represent unknowns.

The goal
To fit a straight line through the standards, and then interpolate concentrations for the three unknown values.

How to fit a dose response curve
1. Click Analyze, choose Linear regression from the list of XY analyses.
2. Check the option:
Interpolate unknowns from standard curve.
3. Look at the graph to make sure the line fits nicely.
4. To find the concentrations corresponding to the unknown values, go to the results subpage: Interpolated X values. Nonlinear standard curve interpolations What if the standard curve isn't linear? saline saline saline saline con saline con saline con IL-1 IL-1 IL-1 IL-1 con IL-1 con IL-1 con Saline Plasma Saline PLasma Saline PLasma IL-1 Plasma IL-1 Plasma IL-1 Plasma Saline EVs Saline EVs Saline EVs IL-1 EVs IL-1 EVs IL-1 EVs Saline Liver Saline Liver Saline Liver IL-1 Liver IL-1 Liver IL-1 Liver 4000 2000 1000 500 250 125 4000 2000 1000 500 250 125 1.338667 0.853 0.514667 0.319667 0.214 0.160333 0.395 0.376 0.4155 0.5665 0.5195 0.4365 0.3285 0.327 0.276 0.311 0.394 0.5065 0.191 0.1205 0.126 0.11 0.123 0.113 0.098 0.107 0.1115 0.1425 0.1085 0.142 0.6325 0.666 0.5965 0.432 0.5665 0.5175 Learn more about interpolating from standard curves How the data are organized
The X values are micrograms of protein. The Y values are the measured optical density. The first seven rows represent standards, where you know both concentration and signal (Y). The next three rows represent unknowns.

The goal
To fit a straight line through the standards, and then interpolate concentrations for the three unknown values.

How to fit a dose response curve
1. Click Analyze, choose Linear regression from the list of XY analyses.
2. Check the option:
Interpolate unknowns from standard curve.
3. Look at the graph to make sure the line fits nicely.
4. To find the concentrations corresponding to the unknown values, go to the results subpage: Interpolated X values. Nonlinear standard curve interpolations What if the standard curve isn't linear? saline saline saline saline con saline con saline con IL-1 IL-1 IL-1 IL-1 con IL-1 con IL-1 con Saline Liver Saline Liver Saline Liver IL-1 Liver IL-1 Liver IL-1 Liver Saline Plasma Saline PLasma Saline PLasma IL-1 Plasma IL-1 Plasma IL-1 Plasma Saline EVs Saline EVs Saline EVs IL-1 EVs IL-1 EVs IL-1 EVs 250 125 62.5 31.25 15.625 7.8125 250 125 62.5 31.25 15.625 7.8125 1.102 0.703667 0.386667 0.236 0.152667 0.115 0.1885 0.219 0.236 0.2565 0.239 0.233 0.6025 0.8555 0.3175 0.238 0.355 0.3085 0.0895 0.101 0.095 0.0925 0.0855 0.0855 0.6695 0.614 0.6555 0.4895 0.716 0.5965 0.0915 0.09 0.088 0.088 0.0845 0.0835 12.9922657640106 21.8217694222473 26.3391898985544 110.424811946181 199.849203647624 50.6716592822997 -16.9869792151187 -20.7857191611042 -20.1697072779714 -19.9643699835938 -19.348358100461 -19.861701336405 -21.0937251026706 -20.375044572349 -20.2723759251602 -20.1697072779714 -13.7015825050771 -19.1430208060834 748.324011026006 685.901473535217 815.674643581858 1311.76954679813 1157.35590142618 884.667974492731 529.845129808243 524.91703474318 357.361802531061 472.350687382515 745.038614315965 1114.64574419564 78.1030821775277 -153.517385880402 -135.447703975174 -188.014051335839 -145.303894105299 -178.157861205714 -227.438811856338 -197.870241465964 -183.085956270776 -81.2386582594881 -192.942146400901 -82.8813566145089 Learn more about interpolating from standard curves How the data are organized
The X values are micrograms of protein. The Y values are the measured optical density. The first seven rows represent standards, where you know both concentration and signal (Y). The next three rows represent unknowns.

The goal
To fit a straight line through the standards, and then interpolate concentrations for the three unknown values.

How to fit a dose response curve
1. Click Analyze, choose Linear regression from the list of XY analyses.
2. Check the option:
Interpolate unknowns from standard curve.
3. Look at the graph to make sure the line fits nicely.
4. To find the concentrations corresponding to the unknown values, go to the results subpage: Interpolated X values. Nonlinear standard curve interpolations What if the standard curve isn't linear? saline saline saline con saline con saline con IL-1 IL-1 IL-1 IL-1 con IL-1 con IL-1 con 10 5 2.5 1.25 0.625 0.3125 10 5 2.5 1.25 0.625 0.3125 0.753 0.643 0.512667 0.217333 0.263 0.306 0.3485 0.335 0.3905 0.373 0.2605 0.284 0.3355 0.2995 0.2925 0.395 Brain Homogenate Plasma EV Fraction 12.9922657640106 -16.9869792151187 -21.0937251026706 21.8217694222473 -20.7857191611042 -20.375044572349 26.3391898985544 -20.1697072779714 -20.2723759251602 110.424811946181 -19.9643699835938 -20.1697072779714 199.849203647624 -19.348358100461 -13.7015825050771 50.6716592822997 -19.861701336405 -19.1430208060834 Brain Homogenate Plasma EV Fraction 748.324011026006 78.1030821775277 -227.438811856338 685.901473535217 -153.517385880402 -197.870241465964 815.674643581858 -135.447703975174 -183.085956270776 529.845129808243 -188.014051335839 -81.2386582594881 524.91703474318 -145.303894105299 -192.942146400901 357.361802531061 -178.157861205714 -82.8813566145089