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The structure of Bacillus subtilis SPβ prophage dUTPase and its complexes with two nucleotides.

Abstract:

dUTPases are housekeeping enzymes which catalyse the hydrolysis of dUTP to dUMP in an ion-dependent manner. Bacillus subtilis has both a genomic and an SPβ prophage homotrimeric dUTPase. Here, structure determination of the prophage apoenzyme and of its complexes with dUDP and dUpNHpp-Mg(2+) is described at 1.75, 1.9 and 2.55 Å resolution, respectively. The C-terminal extension, which carries the conserved motif V, is disordered in all three structures. Unlike all other trimeric dUTPases for ...

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Publication status:
Published

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Publisher copy:
10.1107/s0907444911003234

Authors


García-Nafría, J More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Structural Biology
Persson, R More by this author
Wilson, KS More by this author
Journal:
Acta crystallographica. Section D, Biological crystallography
Volume:
67
Issue:
Pt 3
Pages:
167-175
Publication date:
2011-03-05
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
URN:
uuid:1e2469c7-a7df-4928-9c3f-59fa6d398f75
Source identifiers:
120196
Local pid:
pubs:120196

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