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Nanopore metagenomic sequencing to investigate nosocomial transmission of human metapneumovirus from a unique genetic group among haematology patients in the United Kingdom

Abstract:

Background Human metapneumovirus (HMPV) infection causes a spectrum of respiratory tract disease, and may be a significant pathogen in the context of immunocompromise. Here, we report direct-from-sample metagenomic sequencing of HMPV using Oxford Nanopore Technology.

Methods We applied this sequencing approach to 25 respiratory samples that had been submitted to a clinical diagnostic laboratory in a UK teaching hospital. These samples represented 1...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.jinf.2020.02.003

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Publisher:
Elsevier Publisher's website
Journal:
Journal of Infection Journal website
Volume:
80
Issue:
5
Pages:
571-577
Publication date:
2020-02-22
Acceptance date:
2020-02-08
DOI:
EISSN:
1532-2742
ISSN:
0163-4453
Language:
English
Keywords:
Pubs id:
1087359
Local pid:
pubs:1087359
Deposit date:
2020-02-13

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