Journal article icon

Journal article

Structural analysis of substrate binding by the TatBC component of the twin-arginine protein transport system.

Abstract:

The Tat system transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membrane of plant chloroplasts. In Escherichia coli substrate proteins initially bind to the integral membrane TatBC complex which then recruits the protein TatA to effect translocation. Overproduction of TatBC and the substrate protein SufI in the absence of TatA led to the accumulation of TatBC-SufI complexes that could be purified using an affinity tag on the substrate. Three-dimensional s...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1073/pnas.0901566106

Authors


Journal:
Proceedings of the National Academy of Sciences of the United States of America More from this journal
Volume:
106
Issue:
32
Pages:
13284-13289
Publication date:
2009-08-01
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Language:
English
Keywords:
Pubs id:
pubs:20658
UUID:
uuid:1cc4ca26-23e2-466c-9989-50372db36210
Local pid:
pubs:20658
Source identifiers:
20658
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP