GOAT--a simple LC-MS/MS gradient optimization tool.

Journal article

Modern nano-HPLC systems are capable of extremely precise control of solvent gradients, allowing high-resolution separation of peptides. Most proteomics laboratories use a simple linear analytical gradient for nano-LC-MS/MS experiments, though recent evidence indicates that optimized non-linear gradients result in increased peptide and protein identifications from cell lysates. In concurrent work, we examined non-linear gradients for the analysis of samples fractionated at the peptide level, ...

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Authors: Trudgian, D; Fischer, R; Guo, X; Kessler, B; Mirzaei, H

• Publication status: Published
• Publisher: Wiley
• Journal: Proteomics
• Volume: 14
• Issue: 12
• Pages: 1467-1471
• Publication date: 2014-06-01
• DOI: 10.1002/pmic.201300524
• EISSN: 1615-9861
• ISSN: 1615-9853
• Language: English
• Keywords: Bioinformatics; Gradient; LC-MS/MS; Optimization; Liquid chromatography; Separation