Journal article
Activity-based probes trap early active intermediates during metacaspase activation
- Abstract:
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Metacaspases are essential cysteine proteases present in plants, fungi, and protists that are regulated by calcium binding and proteolytic maturation through mechanisms not yet understood. Here, we developed and validated activity-based probes for the three main metacaspase types, and used them to study calcium-mediated activation of metacaspases from their precursors in vitro. By combining substrate-inspired tetrapeptide probes containing an acyloxymethylketone (AOMK) r...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 2.9MB, Terms of use)
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- Publisher copy:
- 10.1016/j.isci.2022.105247
Authors
Funding
Bibliographic Details
- Publisher:
- Cell Press
- Journal:
- iScience More from this journal
- Volume:
- 25
- Issue:
- 11
- Article number:
- 105247
- Publication date:
- 2022-09-29
- Acceptance date:
- 2022-09-27
- DOI:
- EISSN:
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2589-0042
- Pmid:
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36339259
Item Description
- Language:
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English
- Keywords:
- Pubs id:
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1302993
- Local pid:
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pubs:1302993
- Deposit date:
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2023-08-07
Terms of use
- Copyright holder:
- Štrancar et al
- Copyright date:
- 2022
- Rights statement:
- © 2022 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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