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A rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants.

Abstract:

We describe a green fluorescent protein (GFP)-based assay for investigating membrane traffic on the secretory pathway in plants. Expression of AtRab1b(N121I), predicted to be a dominant inhibitory mutant of the Arabidopsis Rab GTPase AtRab1b, resulted in accumulation of a secreted GFP marker in an intracellular reticulate compartment reminiscent of the endoplasmic reticulum. This accumulation was alleviated by coexpressing wild-type AtRab1b but not AtRab8c. When a Golgi-targeted and N-glycosy...

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Publication status:
Published

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Publisher copy:
10.1105/tpc.12.11.2201

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Journal:
Plant cell
Volume:
12
Issue:
11
Pages:
2201-2218
Publication date:
2000-11-01
DOI:
EISSN:
1532-298X
ISSN:
1040-4651
Source identifiers:
48892

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