Journal article
Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry
- Abstract:
- Membrane proteins reside in lipid bilayers and are typically extracted from this environment for study, which often compromises their integrity. In this work, we ejected intact assemblies from membranes, without chemical disruption, and used mass spectrometry to define their composition. From Escherichia coli outer membranes, we identified a chaperone-porin association and lipid interactions in the β-barrel assembly machinery. We observed efflux pumps bridging inner and outer membranes, and from inner membranes we identified a pentameric pore of TonB, as well as the protein-conducting channel SecYEG in association with F1FO adenosine triphosphate (ATP) synthase. Intact mitochondrial membranes from Bos taurus yielded respiratory complexes and fatty acid–bound dimers of the ADP (adenosine diphosphate)/ATP translocase (ANT-1). These results highlight the importance of native membrane environments for retaining small-molecule binding, subunit interactions, and associated chaperones of the membrane proteome.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Publisher copy:
- 10.1126/science.aau0976
Authors
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science More from this journal
- Volume:
- 362
- Issue:
- 6416
- Pages:
- 829-834
- Publication date:
- 2018-11-16
- Acceptance date:
- 2018-10-08
- DOI:
- EISSN:
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1095-9203
- ISSN:
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0036-8075
- Pmid:
-
30442809
- Language:
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English
- Pubs id:
-
pubs:945352
- UUID:
-
uuid:1c628ad7-e4c9-4ba7-b198-9a12d7bcd405
- Local pid:
-
pubs:945352
- Source identifiers:
-
945352
- Deposit date:
-
2019-01-22
Terms of use
- Copyright holder:
- Chorev et al
- Copyright date:
- 2018
- Notes:
-
Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works http://www.sciencemag.org/about/science-licenses-journal-article-reuse. This is the accepted manuscript version of the article. The final version is available online from American Association for the Advancement of Science at: https://doi.org/10.1126/science.aau0976
Note: a correction exists for this article, originally published and available at: http://dx.doi.org/10.1126/science.aax7485
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