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Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

Abstract:

Membrane proteins reside in lipid bilayers and are typically extracted from this environment for study, which often compromises their integrity. In this work, we ejected intact assemblies from membranes, without chemical disruption, and used mass spectrometry to define their composition. From Escherichia coli outer membranes, we identified a chaperone-porin association and lipid interactions in the β-barrel assembly machinery. We observed efflux pumps bridging inner and outer membranes, and f...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1126/science.aau0976

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Subgroup:
Physical & Theoretical Chem
ORCID:
0000-0002-0751-0092
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Structural Biology
ORCID:
0000-0001-9578-4263
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Subgroup:
Physical & Theoretical Chem
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
More by this author
ORCID:
0000-0002-7115-1565
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Publisher:
American Association for the Advancement of Science Publisher's website
Journal:
Science Journal website
Volume:
362
Issue:
6416
Pages:
829-834
Publication date:
2018-11-16
Acceptance date:
2018-10-08
DOI:
EISSN:
1095-9203
ISSN:
0036-8075
Pubs id:
pubs:945352
URN:
uri:1c628ad7-e4c9-4ba7-b198-9a12d7bcd405
UUID:
uuid:1c628ad7-e4c9-4ba7-b198-9a12d7bcd405
Local pid:
pubs:945352
Language:
English

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