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High-performance liquid chromatography analysis of ganglioside carbohydrates at the picomole level after ceramide glycanase digestion and fluorescent labeling with 2-aminobenzamide.

Abstract:

The functional importance of glycolipids has emphasized the need for more sensitive methods of detection, characterization, and quantification than has often been possible using traditional thin-layer chromatographic techniques. We describe the use of ceramide glycanase and HPLC to identify and quantify gangliosides in which the carbohydrate is in Glcbeta1--> linkage with ceramide. Detection of released carbohydrate was by fluorescent labeling with 2-aminobenzamide at the reducing terminal...

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Publication status:
Published

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Publisher copy:
10.1006/abio.2001.5393

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Journal:
Analytical biochemistry
Volume:
298
Issue:
2
Pages:
207-217
Publication date:
2001-11-01
DOI:
EISSN:
1096-0309
ISSN:
0003-2697
Source identifiers:
100948

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