Journal article
High-performance liquid chromatography analysis of ganglioside carbohydrates at the picomole level after ceramide glycanase digestion and fluorescent labeling with 2-aminobenzamide.
- Abstract:
-
The functional importance of glycolipids has emphasized the need for more sensitive methods of detection, characterization, and quantification than has often been possible using traditional thin-layer chromatographic techniques. We describe the use of ceramide glycanase and HPLC to identify and quantify gangliosides in which the carbohydrate is in Glcbeta1--> linkage with ceramide. Detection of released carbohydrate was by fluorescent labeling with 2-aminobenzamide at the reducing terminal...
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- Publication status:
- Published
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- Publisher copy:
- 10.1006/abio.2001.5393
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Authors
Bibliographic Details
- Journal:
- Analytical biochemistry
- Volume:
- 298
- Issue:
- 2
- Pages:
- 207-217
- Publication date:
- 2001-11-01
- DOI:
- EISSN:
-
1096-0309
- ISSN:
-
0003-2697
- Source identifiers:
-
100948
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:100948
- UUID:
-
uuid:1c51f2c8-b6b5-45d3-b988-c1646963b2f9
- Local pid:
- pubs:100948
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2001
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