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High capacity extrachromosomal gene expression vectors.

Abstract:

Extrachromosomal gene expression vectors that contain native genomic gene expression elements have numerous advantages over traditional integrating mini-gene vectors. In this protocol chapter we describe our work using episomal vectors where expression of a cDNA is controlled by a 10 kB piece of genomic DNA encompassing the promoter of the low density lipoprotein receptor. We explain methods to sub-clone large genomic inserts into gene expression vectors. We also illustrate various methods em...

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Publisher copy:
10.1007/978-1-61779-099-7_2

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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
738
Pages:
19-40
Publication date:
2011-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Source identifiers:
127190

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