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Comprehensive comparison of Pacific Biosciences and Oxford Nanopore Technologies and their applications to transcriptome analysis.

Abstract:

Background: Given the demonstrated utility of Third Generation Sequencing [Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)] long reads in many studies, a comprehensive analysis and comparison of their data quality and applications is in high demand. 

Methods: Based on the transcriptome sequencing data from human embryonic stem cells, we analyzed multiple data features of PacBio and ONT, including error pattern, length, mappability and technica...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.12688/f1000research.10571.2

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Expand authors...
Publisher:
F1000Research Publisher's website
Journal:
F1000Research Journal website
Volume:
6
Pages:
100
Publication date:
2017-06-19
Acceptance date:
2017-06-19
DOI:
EISSN:
2046-1402
ISSN:
2046-1402
Pmid:
28868132
Source identifiers:
724310
Language:
English
Keywords:
Pubs id:
pubs:724310
UUID:
uuid:19d8d0a9-f52d-4a89-aa98-a4274f953b35
Local pid:
pubs:724310
Deposit date:
2017-09-07

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