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ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Abstract:

Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and co...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1186/s13059-021-02302-5

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Role:
Author
ORCID:
0000-0001-9106-3557
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Role:
Author
ORCID:
0000-0003-4816-4103
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Role:
Author
ORCID:
0000-0003-1757-7343
Publisher:
BioMed Central
Journal:
Genome Biology More from this journal
Volume:
22
Issue:
1
Article number:
89
Publication date:
2021-04-08
Acceptance date:
2021-02-19
DOI:
EISSN:
1474-760X
ISSN:
1474-7596
Pmid:
33827654
Language:
English
Keywords:
Pubs id:
1173360
Local pid:
pubs:1173360
Deposit date:
2022-11-30

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