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Measuring nucleotide binding to intact, functional membrane proteins in real time

Abstract:

We have developed a method to measure binding of adenine nucleotides to intact, functional transmembrane receptors in a cellular or membrane environment. This method combines expression of proteins tagged with the fluorescent non-canonical amino acid ANAP, and FRET between ANAP and fluorescent (trinitrophenyl) nucleotide derivatives. We present examples of nucleotide binding to ANAP-tagged KATP ion channels measured in unroofed plasma membranes and excised, inside-out membrane patches under v...

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Publication status:
Published
Peer review status:
Peer reviewed

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Division:
MPLS
Department:
Physics
Sub department:
Condensed Matter Physics
Oxford college:
Green Templeton College
Role:
Author
Publisher:
Journal of Visualized Experiments Publisher's website
Journal:
Journal of Visualized Experiments Journal website
Volume:
169
Article number:
e61401
Publication date:
2021-03-11
Acceptance date:
2020-05-12
DOI:
EISSN:
1940-087X
Language:
English
Keywords:
Pubs id:
1115547
Local pid:
pubs:1115547
Deposit date:
2020-07-02

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