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Journal article

Spinning disk-remote focusing microscopy

Abstract:

Fast confocal imaging was achieved by combining remote focusing with differential spinning disk optical sectioning to rapidly acquire images of live samples at cellular resolution. Axial and lateral full width half maxima less than 5 µm and 490 nm respectively are demonstrated over 130 µm axial range with a 256 × 128 µm field of view. A water-index calibration slide was used to achieve an alignment that minimises image volume distortion. Application to live biological samples was demonstrated...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1364/boe.389904

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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Oxford college:
New College
Role:
Author
ORCID:
0000-0003-1688-259X
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Publisher:
The Optical Society Publisher's website
Journal:
Biomedical Optics Express Journal website
Volume:
11
Issue:
6
Pages:
2874-2874
Publication date:
2020-05-04
Acceptance date:
2020-02-26
DOI:
EISSN:
2156-7085
Language:
English
Keywords:
Pubs id:
1103516
Local pid:
pubs:1103516
Deposit date:
2020-05-11

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