Interleukin-10 gene promoter polymorphism in English and Polish healthy controls. Polymerase chain reaction haplotyping using 3' mismatches in forward and reverse primers.

Journal article

A polymerase chain reaction with sequence-specific primers (PCR-SSP) system using primers with mismatches at the 3' ends was developed to determine polymorphisms in IL-10 promoter region. Three previously described biallelic polymorphisms in IL-10 were linked in a 12 reaction PCR-SSP system and the method used to provide genotype data on 233 UK and 166 Polish controls. There are eight possible polymorphic combinations in IL-10 promoter gene but only three were observed in both control groups. Population frequencies of IL-10 genotypes show, in contrast to HLA, that UK and Polish frequencies are remarkably similar.

Authors: Koss, K; Fanning, G; Welsh, K; Jewell, D

• Publication status: Published
• Journal: Genes and immunity
• Volume: 1
• Issue: 5
• Pages: 321-324
• Publication date: 2000-06-01
• DOI: 10.1038/sj.gene.6363669
• EISSN: 1476-5470
• ISSN: 1466-4879
• Language: English
• Keywords: Interleukin-10; Base Sequence; Polymorphism, Genetic; Alleles; Gene Frequency; Promoter Regions, Genetic; Polymerase Chain Reaction; England; Female; Male; DNA Primers; Humans; Haplotypes; Poland