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Catalytic activity of catalase-silica nanoparticle hybrids: from ensemble to individual entity activity

Abstract:

We demonstrate the electrochemical detection and characterization of individual nanoparticle-enzyme hybrids. Silica nanoparticles were functionalized with catalase enzyme and investigated spectroscopically and electrochemically. The catalytic activity of the hybrids towards hydrogen peroxide decomposition was comparable to the activity of a freely diffusing enzyme in solution, exhibiting a Michaelis-Menten constant of KM = 74 mM and a turnover number of kcat = 8 × 10(7) s(-1) per NP. The fast...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/c6sc04921d

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Institution:
University of Oxford
Oxford college:
Oriel College
Role:
Author
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Institution:
University of Oxford
Oxford college:
St John's College
Role:
Author
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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
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Institution:
University of Oxford
Division:
MPLS
Department:
Materials
Role:
Author
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Funding agency for:
Sepunaru, L
Grant:
FP/2007–2013/ERC320403
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Funding agency for:
Sokolov, S
Grant:
FP/2007–2013/ERC320403
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Funding agency for:
Kätelhön, E
Grant:
FP/2007–2013/ERC320403
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Science Journal website
Volume:
8
Issue:
3
Pages:
2303-2308
Publication date:
2016-12-15
Acceptance date:
2016-12-14
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Source identifiers:
685406
Language:
English
Keywords:
Pubs id:
pubs:685406
UUID:
uuid:12558877-037e-496b-b159-2754818f230f
Local pid:
pubs:685406
Deposit date:
2017-06-07

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