Journal article
Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography
- Abstract:
-
Extracellular vesicles (EVs) play a pivotal role in cell-to-cell communication and have been shown to take part in several physiological and pathological processes. EVs have traditionally been purified by ultracentrifugation (UC), however UC has limitations, including resulting in, operator-dependant yields, EV aggregation and altered EV morphology, and moreover is time consuming. Here we show that commercially available bind-elute size exclusion chromatography (BE-SEC) columns purify EVs wit...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Authors
Funding
+ Swedish Society of Medical Research
More from this funder
Funding agency for:
Andaloussi, S
Grant:
SSMF
Bibliographic Details
- Publisher:
- Springer Nature Publisher's website
- Journal:
- Scientific Reports Journal website
- Volume:
- 7
- Issue:
- 1
- Article number:
- 11561
- Publication date:
- 2017-09-14
- Acceptance date:
- 2017-08-11
- DOI:
- ISSN:
-
2045-2322
- Pmid:
-
28912498
Item Description
- Language:
- English
- Pubs id:
-
pubs:730182
- UUID:
-
uuid:11e6dff3-4d55-4d6c-965f-67a82571b5d5
- Local pid:
- pubs:730182
- Source identifiers:
-
730182
- Deposit date:
- 2017-11-09
Terms of use
- Copyright holder:
- Corso et al
- Copyright date:
- 2017
- Notes:
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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