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Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography

Abstract:

Extracellular vesicles (EVs) play a pivotal role in cell-to-cell communication and have been shown to take part in several physiological and pathological processes. EVs have traditionally been purified by ultracentrifugation (UC), however UC has limitations, including resulting in, operator-dependant yields, EV aggregation and altered EV morphology, and moreover is time consuming. Here we show that commercially available bind-elute size exclusion chromatography (BE-SEC) columns purify EVs wit...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/s41598-017-10646-x

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Görgens, A More by this author
Bultema, J More by this author
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Funding agency for:
Andaloussi, SE
Publisher:
Springer Nature Publisher's website
Journal:
Scientific Reports Journal website
Volume:
7
Issue:
1
Pages:
Article: 11561
Publication date:
2017-09-14
Acceptance date:
2017-08-11
DOI:
ISSN:
2045-2322
Pubs id:
pubs:730182
URN:
uri:11e6dff3-4d55-4d6c-965f-67a82571b5d5
UUID:
uuid:11e6dff3-4d55-4d6c-965f-67a82571b5d5
Local pid:
pubs:730182
Language:
English

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