Journal article
A combined computational and structural model of the full-length human prolactin receptor
- Abstract:
- The prolactin receptor is an archetype member of the class I cytokine receptor family, comprising receptors with fundamental functions in biology as well as key drug targets. Structurally, each of these receptors represent an intriguing diversity, providing an exceptionally challenging target for structural biology. Here, we access the molecular architecture of the monomeric human prolactin receptor by combining experimental and computational efforts. We solve the NMR structure of its transmembrane domain in micelles and collect structural data on overlapping fragments of the receptor with small-angle X-ray scattering, native mass spectrometry and NMR spectroscopy. Along with previously published data, these are integrated by molecular modelling to generate a full receptor structure. The result provides the first full view of a class I cytokine receptor, exemplifying the architecture of more than 40 different receptor chains, and reveals that the extracellular domain is merely the tip of a molecular iceberg.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.0MB, Terms of use)
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(Preview, Version of record, pdf, 860.7KB, Terms of use)
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- Publisher copy:
- 10.1038/ncomms11578
Authors
- Publisher:
- Springer Nature
- Journal:
- Nature Communications More from this journal
- Volume:
- 7
- Article number:
- 11578
- Publication date:
- 2016-01-01
- Acceptance date:
- 2016-04-11
- DOI:
- ISSN:
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2041-1723
- Pmid:
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27174498
- Language:
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English
- Keywords:
- Pubs id:
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pubs:623576
- UUID:
-
uuid:107db79c-d845-451d-a4a8-5b9da99c5338
- Local pid:
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pubs:623576
- Source identifiers:
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623576
- Deposit date:
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2017-04-08
Terms of use
- Copyright holder:
- Bugge et al
- Copyright date:
- 2016
- Notes:
- This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Licence:
- CC Attribution (CC BY)
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