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Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor

Abstract:

Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase in human cells. The crystal structure of the HIV integrase-binding domain (IBD) of LEDGF has been determined in the absence of ligand. IBD was overexpressed in Escherichia coli, purified and crystallized by sitting-drop vapour diffusion. X-ray diffraction data were collected at Diamond Light Source to a resolution of 2.05 Å. The crystals belonged to space group P21, with eight polypeptide chai...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1107/S2053230X18001553

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; Weatherall Insti. of Molecular Medicine
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; Weatherall Insti. of Molecular Medicine
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Structural Biology
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Funding agency for:
Rabbitts, TH
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Funding agency for:
Rabbitts, TH
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Grant:
MR/J000612/1 to Terence H. Rabbitts
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Grant:
12051 to Terence H. Rabbitts
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Grant:
Studentship No. 2008-2011 to Clare Hannon
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Publisher:
International Union of Crystallography Publisher's website
Journal:
Acta Crystallographica Section F: Structural Biology and Crystallization Communications Journal website
Volume:
F74
Issue:
3
Pages:
143-149
Publication date:
2018-02-26
Acceptance date:
2018-01-23
DOI:
ISSN:
1744-3091
Pubs id:
pubs:821934
URN:
uri:0ff72385-6801-4db4-90b4-547106196781
UUID:
uuid:0ff72385-6801-4db4-90b4-547106196781
Local pid:
pubs:821934

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