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Journal article

Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation

Abstract:

Dipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol in terms of membrane partitioning. However, the suitability of TF-Chol for detecting lysosomal cholesterol deposition has recently been questioned. Here, we highlight the fact that the method of lipid delivery and the analysis of time-point both affect the membrane distribution and labeling...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1111/tra.12414

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM - Investigative Medicine Division
Role:
Author
More by this author
Institution:
University of Oxford
Oxford college:
Christ Church
Role:
Author
Publisher:
Wiley Publisher's website
Journal:
Traffic Journal website
Volume:
17
Issue:
9
Pages:
1054-1057
Publication date:
2016-06-13
Acceptance date:
2016-05-13
DOI:
EISSN:
1600-0854
ISSN:
1398-9219
Pmid:
27187581
Source identifiers:
623232
Language:
English
Keywords:
Pubs id:
pubs:623232
UUID:
uuid:0f0a864e-e159-4795-b7e0-f24fa072fa17
Local pid:
pubs:623232
Deposit date:
2017-03-01

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