Journal article icon

Journal article

Use of BODIPY-Cholesterol (TF-Chol) for Visualizing Lysosomal Cholesterol Accumulation.

Abstract:

Dipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol in terms of membrane partitioning. However, the suitability of TF-Chol for detecting lysosomal cholesterol deposition has recently been questioned. Here, we highlight the fact that the method of lipid delivery and the analysis of time-point both affect the membrane distribution and labeling...

Expand abstract
Publication status:
Published

Actions


Access Document


Files:
Publisher copy:
10.1111/tra.12414

Authors


More by this author
Department:
Oxford, MSD, RDM, RDM - Investigative Medicine Division
Role:
Author
More by this author
Department:
Christ Church
Role:
Author

Contributors

Department:
Minerva Foundation Institute for Medical Research, 00290 Helsinki, Finland.
/Academy of Finland More from this funder
/University of Helsinki More from this funder
/Wolfson Foundation More from this funder
Expand funders...
Journal:
Traffic (Copenhagen, Denmark)
Issue:
9
Publication date:
2016-09-05
DOI:
ISSN:
1398-9219 and 1600-0854
Pubs id:
pubs:623232
URN:
uri:0f0a864e-e159-4795-b7e0-f24fa072fa17
UUID:
uuid:0f0a864e-e159-4795-b7e0-f24fa072fa17
Local pid:
pubs:623232

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP