Journal article
Enrichment approach for unbiased sequencing of respiratory syncytial virus directly from clinical samples
- Abstract:
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Background:
Nasopharyngeal samples contain higher quantities of bacterial and host nucleic acids relative to viruses; presenting challenges during virus metagenomics sequencing, which underpins agnostic sequencing protocols. We aimed to develop a viral enrichment protocol for unbiased whole-genome sequencing of respiratory syncytial virus (RSV) from nasopharyngeal samples using the Oxford Nanopore Technology (ONT) MinION platform.Methods:
We a... Expand abstract
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 2.3MB, Terms of use)
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- Publisher copy:
- 10.12688/wellcomeopenres.16756.1
Authors
Funding
Bibliographic Details
- Publisher:
- F1000Research
- Journal:
- Wellcome Open Research More from this journal
- Volume:
- 6
- Article number:
- 99
- Publication date:
- 2021-05-07
- Acceptance date:
- 2024-05-20
- DOI:
- EISSN:
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2398-502X
Item Description
- Language:
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English
- Keywords:
- Pubs id:
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1196725
- Local pid:
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pubs:1196725
- Deposit date:
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2024-05-15
Terms of use
- Copyright holder:
- Waweru et al
- Copyright date:
- 2021
- Rights statement:
- © 2021 Waweru JW et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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