Journal article icon

Journal article

Rapid high-resolution measurement of DNA replication timing by droplet digital PCR

Abstract:

Genomes are replicated in a reproducible temporal pattern. Current methods for assaying allele replication timing are time consuming and/or expensive. These include high-throughput sequencing which can be used to measure DNA copy number as a proxy for allele replication timing. Here, we use droplet digital PCR to study DNA replication timing at multiple loci in budding yeast and human cells. We establish that the method has temporal and spatial resolutions comparable to the high-throughput se...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

Actions


Access Document


Files:
Publisher copy:
10.1093/nar/gky590

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences
Department:
Pathology Dunn School
Role:
Author
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
2018
Issue:
1
Chapter number:
gky590
Publication date:
2018-07-09
Acceptance date:
2018-06-18
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Pubs id:
pubs:865258
URN:
uri:0cbd5d52-d0eb-4314-b5f8-f1e91b8503c5
UUID:
uuid:0cbd5d52-d0eb-4314-b5f8-f1e91b8503c5
Local pid:
pubs:865258

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP