Genomes are replicated in a reproducible temporal pattern. Current methods for assaying allele replication timing are time consuming and/or expensive. These include high-throughput sequencing which can be used to measure DNA copy number as a proxy for allele replication timing. Here, we use droplet digital PCR to study DNA replication timing at multiple loci in budding yeast and human cells. We establish that the method has temporal and spatial resolutions comparable to the high-throughput se...Expand abstract
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- DG Batrakou et al.
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Copyright The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.
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Rapid high-resolution measurement of DNA replication timing by droplet digital PCR
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