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A high-sensitivity HPLC assay for measuring intracellular Na(+) and K(+) and its application to Plasmodium falciparum infected erythrocytes.

Abstract:
The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na(+) and K(+) content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/srep29241

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Tropical Medicine
Role:
Author
ORCID:
0000-0002-8529-9317
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Role:
Author
ORCID:
0000-0002-5613-2622
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Name:
German Academic Exchange Service
More from this funder
Name:
Australian National Health and Medical Research Council
Publisher:
Nature Publishing Group
Journal:
Scientific Reports More from this journal
Volume:
6
Issue:
1
Pages:
29241
Publication date:
2016-07-07
Acceptance date:
2016-06-14
DOI:
EISSN:
2045-2322
ISSN:
2045-2322
Pmid:
27385291
Language:
English
Keywords:
Pubs id:
pubs:629980
UUID:
uuid:0c77387f-1155-42ed-8f56-252d35b2dbd9
Local pid:
pubs:629980
Source identifiers:
629980
Deposit date:
2018-01-22

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