Journal article
Total fatty acid analysis of human blood samples in one minute by high-resolution mass spectrometry
- Abstract:
- Total fatty acid analysis is a routine method in many areas, including lipotyping of individuals in personalized medicine, analysis of foodstuffs, and optimization of oil production in biotechnology. This analysis is commonly done by converting fatty acyl (FA) chains of intact lipids into FA methyl esters (FAMEs) and monitoring these by gas-chromatography (GC)-based methods, typically requiring at least 15 min of analysis per sample. Here, we describe a novel method that supports fast, precise and accurate absolute quantification of total FA levels in human plasma and serum samples. The method uses acid-catalyzed transesterification with 18O-enriched H2O (i.e., H218O) to convert FA chains into 18O-labeled free fatty acids. The resulting “mass-tagged” FA analytes can be specifically monitored with improved signal-to-background by 1 min of high resolution Fourier transform mass spectrometry (FTMS) on an Orbitrap-based mass spectrometer. By benchmarking to National Institute of Standards and Technology (NIST) certified standard reference materials we show that the performance of our method is comparable, and at times superior, to that of gold-standard GC-based methods. In addition, we demonstrate that the method supports the accurate quantification of FA differences in samples obtained in dietary intervention studies and also affords specific monitoring of ingested stable isotope-labeled fatty acids (13C16-palmitate) in normoinsulinemic and hyperinsulinemic human subjects. Overall, our novel high-throughput method is generic and suitable for many application areas, spanning basic research to personalized medicine, and is particularly useful for laboratories equipped with high resolution mass spectrometers, but lacking access to GC-based instrumentation.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, zip, 370.1KB, Terms of use)
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- Publisher copy:
- 10.3390/biom9010007
Authors
- Publisher:
- MDPI
- Journal:
- Biomolecules More from this journal
- Volume:
- 9
- Issue:
- 1
- Article number:
- 7
- Publication date:
- 2018-12-27
- Acceptance date:
- 2018-12-17
- DOI:
- EISSN:
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2218-273X
- ISSN:
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2218-273X
- Pmid:
-
30591667
- Language:
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English
- Keywords:
- Pubs id:
-
pubs:959249
- UUID:
-
uuid:0950d9ef-356f-4061-9182-5e0723eef7a7
- Local pid:
-
pubs:959249
- Source identifiers:
-
959249
- Deposit date:
-
2019-02-12
Terms of use
- Copyright holder:
- Gallego et al
- Copyright date:
- 2018
- Notes:
- © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). The final version of this article is available online with supplementary materials from MDPI at: https://doi.org/10.3390/biom9010007
- Licence:
- CC Attribution (CC BY)
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