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Profiling RNA polymerase II phosphorylation genome-wide in fission yeast

Abstract:

The RNA polymerase II carboxyl-terminal domain (CTD) consists of tandem repeats of consensus sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Dynamic posttranslational modifications of the CTD generate a CTD code crucial for the cotranscriptional recruitment of factors that control transcription, chromatin modification, and RNA processing. Analysis of CTD phosphorylation by ChIP (Chromatin ImmunoPrecipitation) coupled with high-throughput DNA sequencing (ChIP-seq) is a powerful tool to investiga...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/bs.mie.2018.07.009

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Name:
Wellcome Trust
Grant:
106994/Z/15/Z
Publisher:
Elsevier
Host title:
Methods in Enzymology
Volume:
612
Issue:
Chapter 20
Pages:
489-504
Publication date:
2018-08-23
DOI:
EISSN:
1557-7988
ISSN:
0076-6879
Pmid:
30502955
ISBN:
9780128159934
Language:
English
Keywords:
Pubs id:
pubs:911644
UUID:
uuid:0849e307-9306-47d9-b704-efaf6c9285e9
Local pid:
pubs:911644
Source identifiers:
911644
Deposit date:
2019-07-11

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