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Microsecond resolution of single-molecule rotation catalyzed by molecular motors.

Abstract:

Single-molecule measurements of rotation catalyzed by the F(1)-ATPase or the F(o)F(1) ATP synthase have provided new insights into the molecular mechanisms of the F(1) and F(o) molecular motors. We recently developed a method to record ATPase-driven rotation of F(1) or F(o)F(1) in a manner that solves several technical limitations of earlier approaches that were significantly hampered by time and angular resolution, and restricted the duration of data collection. With our approach it is possi...

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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
778
Pages:
273-289
Publication date:
2011-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:08223815-61fb-4c76-960a-f1d643171f65
Source identifiers:
167774
Local pid:
pubs:167774

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