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Protein film infrared electrochemistry demonstrated for study of H2 oxidation by a [NiFe] hydrogenase

Abstract:

Understanding the chemistry of redox proteins demands methods that provide precise control over redox centers within the protein. The technique of protein film electrochemistry, in which a protein is immobilized on an electrode surface such that the electrode replaces physiological electron donors or acceptors, has provided functional insight into the redox reactions of a range of different proteins. Full chemical understanding requires electrochemical control to be combined with other techni...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3791/55858

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
More from this funder
Funding agency for:
Ash, P
Grant:
BB/L009722/1
BB/N006321/1
More from this funder
Funding agency for:
Ash, P
Grant:
BB/L009722/1
BB/N006321/1
More from this funder
Funding agency for:
Ash, P
Grant:
BB/L009722/1
BB/N006321/1
More from this funder
Funding agency for:
Vincent, K
Grant:
BB/L009722/1
BB/N006321/1
More from this funder
Funding agency for:
Vincent, K
Grant:
BB/L009722/1
BB/N006321/1
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Publisher:
Journal of Visualized Experiments Publisher's website
Journal:
Journal of Visualized Experiments Journal website
Issue:
130
Article number:
e55858
Publication date:
2017-12-04
Acceptance date:
2017-03-17
DOI:
ISSN:
1940-087X
Source identifiers:
689692

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