Book section
Interrogation of functional miRNA–target interactions by CRISPR/Cas9 genome engineering
- Abstract:
-
Post-transcriptional silencing by microRNAs (miRNAs) is a critical constituent of eukaryotic gene regulation. miRNAs are short (~22nt) noncoding RNAs capable of specifically targeting the miRNA-induced-silencing-complex (miRISC) to transcripts bearing a complementary miRNA response element (MRE). Although recent methodological advances have greatly improved our understanding of miRNA biogenesis and the mechanisms by which miRNAs repress their cognate targets, exploring the physiological relev...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Bibliographic Details
- Publisher:
- Humana Press Publisher's website
- Host title:
- Methods in Molecular Biology
- Series:
- Methods in Molecular Biology
- Volume:
- 1580
- Pages:
- 79-97
- Publication date:
- 2017-04-25
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
978-1-4939-6864-0 and 1064-3745
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:692311
- UUID:
-
uuid:07000023-97e5-457b-b9e7-aa0bd0f298ec
- Local pid:
- pubs:692311
- Source identifiers:
-
692311
- Deposit date:
- 2017-08-13
Terms of use
- Copyright holder:
- Springer Science
- Copyright date:
- 2017
- Notes:
- Copyright © Springer Science+Business Media LLC 2017.
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