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Identification of RNA-binding domains of RNA-binding proteins in cultured cells on a system-wide scale with RBDmap

Abstract:

RBDmap identifies, in a proteome-wide manner, the regions of RNA-binding proteins (RBPs) engaged in native interactions with RNA. In brief, cells are irradiated with UV light to induce protein-RNA crosslinks. Resulting covalently linked protein-RNA complexes are purified with oligo(dT) magnetic beads, following stringent denaturing washes. After elution, RBPs are subjected to partial proteolysis, where the protein regions still bound to the RNA and those released to the supernatant are separa...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/nprot.2017.106

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Funding agency for:
Castello, A
Grant:
MR/L019434/1
Publisher:
Springer Nature Publisher's website
Journal:
Nature Protocols Journal website
Volume:
12
Issue:
12
Pages:
2447–2464
Publication date:
2017-11-02
Acceptance date:
2017-07-13
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Source identifiers:
709367
Keywords:
Pubs id:
pubs:709367
UUID:
uuid:06221d07-006f-4cc0-85ee-32bc070c2ade
Local pid:
pubs:709367
Deposit date:
2017-07-27

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