Journal article
Identification of RNA-binding domains of RNA-binding proteins in cultured cells on a system-wide scale with RBDmap
- Abstract:
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RBDmap identifies, in a proteome-wide manner, the regions of RNA-binding proteins (RBPs) engaged in native interactions with RNA. In brief, cells are irradiated with UV light to induce protein-RNA crosslinks. Resulting covalently linked protein-RNA complexes are purified with oligo(dT) magnetic beads, following stringent denaturing washes. After elution, RBPs are subjected to partial proteolysis, where the protein regions still bound to the RNA and those released to the supernatant are separa...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Accepted manuscript, pdf, 25.3MB)
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- Publisher copy:
- 10.1038/nprot.2017.106
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Authors
Funding
+ Medical Research Council
More from this funder
Funding agency for:
Castello, A
Grant:
MR/L019434/1
Bibliographic Details
- Publisher:
- Springer Nature Publisher's website
- Journal:
- Nature Protocols Journal website
- Volume:
- 12
- Issue:
- 12
- Pages:
- 2447–2464
- Publication date:
- 2017-11-02
- Acceptance date:
- 2017-07-13
- DOI:
- EISSN:
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1750-2799
- ISSN:
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1754-2189
- Source identifiers:
-
709367
Item Description
- Keywords:
- Pubs id:
-
pubs:709367
- UUID:
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uuid:06221d07-006f-4cc0-85ee-32bc070c2ade
- Local pid:
- pubs:709367
- Deposit date:
- 2017-07-27
Terms of use
- Copyright holder:
- Castello et al
- Copyright date:
- 2017
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Springer Nature at: https://doi.org/10.1038/nprot.2017.106
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