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Transverse cardiac slicing and optical imaging for analysis of transmural gradients in membrane potential and Ca2+ transients in murine heart

Abstract:

Transmural and regional gradients in membrane potential and Ca2+ transient in the murine heart are largely unexplored. Here, we developed and validated a robust approach which combines transverse ultra‐thin cardiac slices and high resolution optical mapping to enable systematic analysis of transmural and regional gradients in transmembrane potential (Vm) and intracellular Ca2+ transient (CaT) across the entire murine ventricles. The voltage dye RH237 or Ca2+ dye Rhod‐2 AM were loaded through ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1113/JP276239

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Institution:
University of Oxford
Division:
Medical Sciences
Department:
Pharmacology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences
Department:
Pharmacology
Role:
Author
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Publisher:
John Wiley & Sons Publisher's website
Journal:
Journal of Physiology Journal website
Volume:
596
Issue:
17
Pages:
3951-3965
Publication date:
2018-06-21
Acceptance date:
2018-06-12
DOI:
EISSN:
1469-7793
ISSN:
0022-3751
Pubs id:
pubs:858761
URN:
uri:05fce754-d1fe-4b00-a8a5-35c50a1e049f
UUID:
uuid:05fce754-d1fe-4b00-a8a5-35c50a1e049f
Local pid:
pubs:858761

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