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When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping

Abstract:

Background Genome editing in mice using either classical approaches like homologous recombination or CRISPR/Cas9 has been reported to harbor off target effects (insertion/deletion, frame shifts or gene segment duplications) that lead to mutations not only in close proximity to the target site but also outside. Only the genomes of few engineered mouse strains have been sequenced. Since the role of the ether-lipid cleaving enzyme alkylglycerol monooxygenase (AGMO) in physiology and pathophysiol...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1186/s13578-021-00566-9

Authors


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Role:
Author
ORCID:
0000-0002-6375-5393
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Role:
Author
ORCID:
0000-0001-5677-8979
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Role:
Author
ORCID:
0000-0003-3260-9139
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Role:
Author
ORCID:
0000-0003-0971-9858
Publisher:
BioMed Central
Journal:
Cell and Bioscience More from this journal
Volume:
11
Article number:
54
Place of publication:
England
Publication date:
2021-03-16
Acceptance date:
2021-02-25
DOI:
EISSN:
2045-3701
Pmid:
33726865
Language:
English
Keywords:
Pubs id:
1169651
Local pid:
pubs:1169651
Deposit date:
2021-07-13

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