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Identifying mixed Mycobacterium tuberculosis infection and laboratory cross-contamination during mycobacterial sequencing programs

Abstract:

Introduction: Detecting laboratory cross-contamination and mixed tuberculosis infection are important goals of clinical Mycobacteriology laboratories. Objectives: To develop a method detecting mixtures of different M. tuberculosis lineages in laboratories performing Mycobacterial next generation sequencing (NGS). Setting: Public Health England National Mycobacteriology Laboratory Birmingham, which performs Illumina sequencing on DNA extracted from positive Mycobacterial Growth Indicator tubes...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1128/JCM.00923-18

Authors


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Institution:
University of Oxford
Division:
Medical Sciences
Department:
RDM; Clinical Laboratory Sciences
Robinson, E More by this author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences
Department:
Experimental Medicine
More by this author
Institution:
University of Oxford
Division:
Medical Sciences
Department:
RDM; Clinical Laboratory Sciences
Ajileye, A More by this author
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National Institute for Health Research More from this funder
Publisher:
American Society for Microbiology Publisher's website
Journal:
Journal of Clinical Microbiology Journal website
Volume:
56
Issue:
11
Pages:
1-11
Publication date:
2018-09-12
Acceptance date:
2018-08-28
DOI:
EISSN:
1098-660X
ISSN:
0095-1137
Pubs id:
pubs:910891
URN:
uri:05025cb9-824a-4936-b14e-f599b69b1f73
UUID:
uuid:05025cb9-824a-4936-b14e-f599b69b1f73
Local pid:
pubs:910891

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